The VacuCon® Concentrator offers simple and fast ultrafiltration concentration of samples greater than 50mL. This breakthrough device can quickly concentrate 50–200mL to very high concentration factors, up to 100-fold. All you need to operate the VacuCon® concentrator is a conventional vacuum source!
Walk-away operation means that once you add your sample to the feed reservoir and vacuum is connected, you can walk away from the concentrator – the device self-primes and terminates permeation automatically, releasing you from having to constantly observe the concentration process. When you return to the device, just disconnect the vacuum and recover the retentate fraction or concentrate into a syringe.
Buffer exchange or washing of the retentate in situ is possible in a subsequent operation with the VacuCon® system.
VacuCon Concentrator Performance Summary
Easy to use
Recover concentrate via syringe
Concentrated sample ends up in a dedicated receptable suitable for dispensing where desired
No additional hardware required
Up to a 100x concentration factor
Over 85% recovery
Under 1 hour
VacuCon® Concentrator Process
Fast Permeation Means Getting More Experiments Done
Performance: Concentration and Recovery
|Sample||Sample Conc.||Sample Vol.||Recovery Vol.||Yield||Approx. Time*||Vol. Conc. Factor|
95+% Protein recovery in a 3mL recovery volume
Today's Lab Ultrafiltration for 50∼200mL: Not Fast…Not Easy
Concentration at the laboratory scale for volumes from 50mL to 1L isn't fast or easy. When a lab preparative sample of relatively large volume (e.g., 100mL) needs to be concentrated, it either needs to be split among several centrifugal concentrators or a complicated scaled-down TFF process system needs to be configured in the lab bench.
The centrifugal approach requires (a) a large centrifuge (not available in all laboratories) and (b) that the sample be split into multiple aliquots. The permeation is slow, resulting in a long spin time in addition to the time and effort spent handling the splitting and recombining aliquots. Finally, there is limited control over the recovery volumes and concentrations.
The scaled down process systems, such as miniature TFF systems, are complicated to configure. They require expensive hardware in pumps and valves. There is also an upper limit on the final concentration dictated by the system volume.
A Breakthrough Sample Concentrator for Large Samples
Ultrafiltration with walk-away operation
Concentrate 50–100mL down to 1mL in less than 1 hour
Faster, simpler sample processing than centifugal concentrators or scaled-down TFF systems
No additional hardware required: all you need is a source of vacuum
Optional buffer exchange in situ is possible
Optional recovery of the permeate
VacuCon® is the optimal solution for:
BSA Concentration: concentrate 1mg∕mL to 100x
Extracellular proteins and enzymes: concentrate 0.1mg∕mL to 100x
IgG: up to 10mg∕mL
Glycerol removal from antibodies, in lieu of overnight dialysis
DNA and RNA in gene therapy development
…and many more!
Try It Out!
Fill out the form below to be contacted by our applications team and request a VacuCon® evaluation sample!